Putrescine uptake in cystic fibrosis fibroblasts [letter].

Our laboratory has reported abnormal polyamine levels in extracted whole blood from 
individuals with cystic fibrosis (CF). Since an abnormality in putrescine uptake 
in CF fibroblasts would imply a direct relationship to the genetic 
lesion, we initiated studies to confirm and possible extend this observation. 
There is no significant increase in the Vmax of CF fibroblasts 
as compared to values obtained for control cells. We conclude that 
with our growth and assay conditions, in which we attempted to 
duplicate those described in the original investigation, there is no consistent 
or significant increase in the Vmax of putrescine uptake in CF 
fibroblasts. 