Adenosine 3':5'-cyclic AMP in fibroblasts from patients with cystic fibrosis and its relationship to secretion.

Because of the known involvement of cyclic AMP in the control of 
secretion in exocrine and other mammalian cells, we have studied cyclic 
AMP metabolism in cultured cystic fibrosis cells in the hopes of 
demonstrating such an abnormality. A detailed comparison of 5 CF and 
5 control strains showed that the CF cells synthesized 3 times 
more intracellular cyclic AMP than controls. In this paper, we present 
data on an additional 10 CF and 10 control strains. Measurement 
of intracellular cyclic AMP did not provide the same clear-cut differences 
between the CF and control strains, as we had previously observed. 
The CF strains synthesized more cyclic AMP than controls but the 
difference was not as large as in our earlier study and 
there is considerable overlap between the two groups. It is not 
clear why these two studies should have yielded such disparate results. 
