Electrophoretic separation, detection, and variation of amylase isoenzymes.

An electrophoretic technique for demonstrating amylase isoenzymes is described. After separation in 
an agarose gel containing a linear polyacrylamide polymer to reduce electroendosmotic 
flow, the amylase fractions are visualized by incubation with a commercially 
available dye-starch polymer (Phadebas Amylase Test). The technique detects amylase fractions 
with activities below 10 U/l. Some characteristic changes in such diseases 
as acute and chronic pancreatitis, cystic fibrosis of the pancreas, macroamylasemia 
and inherited variants as well as after maxillofacial surgery are mentioned. 
