Mucoid strains of Pseudomonas aeruginosa: the influence of culture medium on the stability of mucus production.

Mucoid strains of Pseudomonas aeruginosa isolated from the respiratory tract of patients 
with cystic fibrosis (CF), those obtained from non-CF patients, and those 
obtained in vitro by the action of phage, were found to 
be stable in their mucoid colonial form when serially subcultured on 
deoxycholate-citrate agar. The ability of anionic, cationic and neutral surfactants to 
stabilise mucus production is described. The possible importance of dipalmitoyl lecithin 
as a stabilising agent for mucus production in vivo is considered, 
with particular reference to the role of mucoid P. aeruginosa in 
CF disease. 