A simple method for the quantitative determination of stool trypsin and chymotrypsin.

A simple method for the determination of the activity of trypsin and 
chymotrypsin in stool and duodenal aspirations is described. The method is 
based on that described by Barbero et al. (Am. J. Dis. 
Child., 112 (1966) 536) [1] but utilizes the time for a 
drop of 0.1 pH unit in the solution, instead of a 
constant pH titration, to determine enzyme activity. Results for a number 
of cystic fibrosis patients and normals compare favorably with the literature. 
