Glycosidases in human skin fibroblast cultures. Alpha-fucosidase, alpha-galactosidase, alpha-glucosidase, beta-mannosidase, and N-acetyl-alpha-glucosaminidase.

Five glycosidases, alpha-fucosidase, alpha-galactosidase, alpha- glucosidase, beta-mannosidase and N-acetyl-alpha-glucosaminidase were studied in 
human skin fibroblast cultures. The pH-dependency, kinetic properties of the enzymes 
and results of isoelectric focusing and ion exchange chromatography are presented. 
Techniques suitable for diagnosing inborn lysosomal diseases in skin fibroblast cultures 
are defined. 