The effect of cystic fibrosis and non-cystic fibrosis plasma on electrophysiological responses in isolated frog gastric mucosa.

An alternative method for assaying the cystic fibrosis factor could be on 
the electrophysiological responses of isolated living tissues. The ability of the 
frog's gastric mucosa to secrete hydrochloric acid and maintain a negative 
potential difference is well known. This potential difference across the secretory 
(mucosa) to the nutrient (serosa) side coupled with its property of 
transporting chloride and hydrogen ions against an electrochemical gradient could therefore 
serve as a useful model for investigating any induced changes in 
the presence of CF and non-CF plasma. From the results obtained 
the short circuit current and H+ secretion responses in the presence 
of CF (whole) plasma show significant increases compared to the control. 
These findings show that in the presence of CF plasma, whole 
or plasma fraction B, there is a marked increase in the 
actively transported Cl- and H+. The effect of CF plasma in 
increasing net Cl- flux is important considering the elevation of this 
electrolyte in the sweat of CF patients and deserved further investigation. 
