Animal model for cystic fibrosis: pulmonary clearance of Staphylococcus aureus in mice treated with reserpine.

Since it has been demonstrated that chronic administration of reserpine to rats 
produces CF-like alterations, we have studied the lung resistance to bacterial 
infections in animals treated with reserpine. Mice of the BALB/c inbred 
strain were injected subcutaneously with different doses of reserpine for 7 
days; after, they were submitted to an infective aerosol containing Staphylococcus 
aureus in a nebulization chamber. With each pair of exposed animals, 
an individual value of the uncleared bacteria ratio (UBR) at 4 
hours was obtained. Reserpinized animals showed a significant increase in UBR 
values when compared to control mice (p < 0.05 and p 
< 0.01 depending on the doses), suggesting an impairment of the 
lung antibacterial defenses. Although pharmacological doses of reserpine produce catecholamine depletion, 
we can not conclude that this action upon the nervous system 
is the only cause of the CF-like response we detected. In 
conclusion, the observed UBR decrease lends support to the concept of 
a reserpine- induced CF animal model. 