Detection of cystic fibrosis with plasma hydrolases [letter].

Dr Hosli and Mrs Hosli reported that differential thermolability of plasma alpha-mannosidase 
and plasma acid phosphatase allowed discrimination between normal people, cystic fibrosis 
carriers, and patients. We have repeated these experiments, paying special attention 
to the technical details given in the Hoslis's paper. In a 
separate series of experiments we used larger volumes of plasma for 
each assay, which was performed either with or without non-fluorescent paraffin 
oil. In both cases the blood samples were processed exactly as 
outlined in Hosli and Vogt's paper. There were no significant differences 
in the heat stability of plasma alpha-mannosidase and acid phosphatase between 
the three groups. 